Figure 4.

Tracing of selected Golgi stacks over 30 s. A, Cortical region in untreated cell. B, Transvacuolar strands in untreated cell. C, Cortical region in cell treated with 40 μm cytochalasin D for 30 min. D, Cortical region in cell treated with 3.3 μm nocodazole for 30 min. The positions of individual Golgi stacks are marked in 1-s intervals. The tracings of the stacks are color coded according to the streaming coefficient of movement over the entire observation period (see text). Tracings in warm colors (red and yellow) represent stacks with a high degree of streaming, tracing in cold colors (blue) represent stacks that are mostly wiggling (compare color scale in C). Note that streaming stacks follow straight, preferred paths. Arrows denote the direction of movement. Cytochalasin D treatment eliminated streaming (C), while nocodazole did not affect directed movement (D). Video sequences can be viewed at http://www.plantphysiol.org/cgi/content/full/121/4/1127/DC1.