Table II.
Fraction of cells with active streaming in a population (%)
Experiment | 3.3 μm Nocodazole | Control | Ratio |
---|---|---|---|
1 | 81 | 74 | 1.09 |
2 | 83 | 82 | 1.01 |
3 | 84 | 84 | 1.00 |
4 | 77 | 64 | 1.20 |
5 | 73 | 64 | 1.14 |
6 | 78 | 57 | 1.37 |
7 | 68 | 60 | 1.13 |
8 | 81 | 78 | 1.04 |
9 | 53 | 46 | 1.15 |
10 | 61 | 56 | 1.09 |
Mean ± sd | 74 ± 11 | 67 ± 13 | 1.12 ± 0.11 |
Aliquots of a suspension cultures were treated with 3.3 μm nocodazole or 0.1 % (w/v) DMSO (control). After about a 45-min incubation, 100 cells per treatment were classified as displaying active streaming or not. Ten independent experiments were performed. While the average fraction of cells displaying active streaming was not significantly changed by the drug treatment, a consistent small increase was observed in most experiments (P = 0.004, paired t test).