TRL, RAGE, and IL-1 all initiate their inflammatory effects though MYD88 with subsequent action though members of the IRAK family that activate TRAF6. TNF-α can simulate the cell in both its soluble and membrane bound form by activating TNFR1 and TNFR2 respectively. TNFR1 ligand binding results in a conformational change leading to the recruitment of TRADD, RIP1, TRAF2, and CIAP1/2. Ligand binding to TNFR2 results in the recruitment of similar down stream factors. All five of these receptor pathways converge on NF-κβ and MAPK activation to induce their inflammatory and catabolic effects.