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. Author manuscript; available in PMC: 2019 May 11.
Published in final edited form as: Circ Res. 2018 Feb 26;122(10):e75–e83. doi: 10.1161/CIRCRESAHA.117.312082

Figure 4. Amyloid accumulation in pressure overload HF as detected non-invasively by Positron Emission Tomography (PET) and ex vivo by ThT in-gel stain.

Figure 4

Four-week TAC mice and sham controls were injected with the amyloid PET-tracer Amyvid and imaged by PET. Representative images of the tracer signal, superimposed on CT scans are provided (A top). The SPECT tracer Cardiolite was used to rule out difference in myocardial blood flow. Normalized Amyvid uptake was analyzed (N=8 each, A bottom). Thioflavin T (ThT) in-gel stain confirmed a ≈3-fold increase in short, fibrillar amyloid ex-vivo (P=0.0005, N=6 each, C). The presence of desmin in these bands was confirmed by MS (Online Figure V). A ≈21% decrease in the ThT signal upon treatment with epigallocatechin gallate (EGCG) confirmed the specificity of the stain (C–D).