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. Author manuscript; available in PMC: 2019 May 11.
Published in final edited form as: Circ Res. 2018 Feb 26;122(10):1347–1353. doi: 10.1161/CIRCRESAHA.117.312215

Figure 3. Binding partners.

Figure 3

(A) Silver-stained SDS-PAGE gel after RNA pull-down assay. The band specific to the sense sample but missing in the antisense sample was used for mass spectrometry analysis. In addition, the similar region in the antisense sample was analyzed by mass spectrometry. As result, 9 proteins listed were detected only in the sense Airn-001 compared to antisense one (negative control) in two idependent assays. (B, C) Western blotting after RNA pull-down assay for (B) Rpa1; and (C) Igf2bp2 “M” stands for marker; “I” for 5% input; “A” for antisense; and “S” for sense probe for Airn-001. Representative image from 5 independent assays. (D) RIP using anti-Igf2bp2 antibody. The expression values were normalized to those of the input. * and ** represent p<0.05 and 0.01, respectively. n=3.