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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Traffic. 2018 Apr 24;19(6):463–480. doi: 10.1111/tra.12564

Figure 7. Retrograde trafficking is impaired in COG KO cells but not MGAT1 or GALE.

Figure 7

Cells were treated with Subtilase cytotoxin (SubAB) for the indicated time points at 37°C then collected and lysed. Samples were analysed by WB (A, FL= Full length GRP78) and quantified using fluorescent densitometry for cleavage of SubAB target protein GRP78/Bip (B). SubAB takes ~60 minutes longer to reach the ER and cleave GRP78 in COG KO cells than MGAT1 or GALE KO cells. (n=2) * denotes significance from MGAT1 values # denotes significance from GALE values. MGAT1 and GALE were not statistically significant from one another. P < 0.05. Error bars represent standard deviation.