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. 2018 May 11;9(5):558. doi: 10.1038/s41419-018-0617-7

Fig. 1. Caspase-3 deficiency promoted DEN-induced liver cancer.

Fig. 1

a CASPASE-3, active CASPASE-3 and β-actin protein expression in the livers of WT mice 3 days following injection with either saline (Un) or 100 mg/kg of DEN was analyzed by western blotting. Values are means ± SD, n = 3 mice in each group. b Active CASPAPS-3 protein (red) in the livers of WT and Caspase-3 KO mice following indicated treatment was detected by IF with nuclei counterstained with DAPI (magnification, ×400). c Photographs of livers of WT and Caspase-3 KO mice 9 months after DEN injection. d Quantification of liver tumor numbers (n = 6). e Quantification of liver tumor sizes (n = 6). f Histological analysis (H&E staining) and staining of AFP of the livers of WT and Caspase-3 KO mice 9 months after DEN injection. Bars: 20 µM. Values in (d) and (e) are means ± SDs