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. 2018 May 11;9(5):564. doi: 10.1038/s41419-018-0618-6

Fig. 2. Genomic structure of PRC1, PRC1 expression in OSCC cell lines and its knockdown in HSC-2 and Cal-27 cells.

Fig. 2

a Genomic structure of PRC1 variants 1, 2, and 4. Gray boxes indicate protein-coding regions while white boxes indicate non-coding regions. RNAi-22440-1 locates in common exon 6 and primers in common exon 11. Genomic information was obtained from the NCBI database (V1: Gene, NM_003981.3; V2: Gene, NM_199413.2; V4: Gene, NM_001267580.1). b Higher PRC1 mRNA expression levels were observed in HSC-2, FaDu, and Cal-27 cells than other OSCC cell lines or OKF-4 cells. c, d PRC1 expression was high in HSC-2, Cal-27, and SCC-9 cells as observed using western blot analysis. e–h Combined RT-PCR and western blot analyses demonstrated that a PRC1-RNAi sequence (22440-1) can effectively downregulate PRC1 expression in HSC-2 and Cal-27 cells. GAPDH was used to normalize RT-PCR results, and as a loading control in western blots. All n = 3; error bars, mean ± SD; n.s, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; t-test