Fig. 5. PRC1 knockdown activates p53 and inhibits the EGFR signaling pathway.

a, e, f After PRC1 knockdown, TβRII and AURKA expression in HSC-2 cells and AURKA expression in Cal-27 cells were unaltered while TβRII was decreased in Cal-27 cells. b, e, f Expression of pho-p53 (Ser³⁹²) increased while EGFR decreased significantly following PRC1 knockdown. c, g, h Following activation with Nutlin-3, expression of both p53 and pho-p53 (Ser³⁹²) increased while that of PRC1 and EGFR decreased significantly. d, i, j P53 and pho-p53 (Ser³⁹²) were inhibited by Pifithrin-α, which in turn initiated an upregulation of both PRC1 and EGFR. All n ≥ 3; error bars, mean ± SD; n.s, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; t-test