Fig. 2. LncRNA-PRLB knockdown inhibited cell proliferation and metastasis in vitro.

a The knockdown of lncRNA-PRLB in MDA-MB-231, MDA-MB-468, and MCF7 cells was validated with qRT-PCR. b Effects of lncRNA-PRLB knockdown on the proliferation of MDA-MB-231, MDA-MB-468, and MCF7 cells were examined with MTT assay. Experiments were performed in triplicate. c Flow cytometry was performed to determine the effect of lncRNA-PRLB on changes of cell cycle distribution. The data represent the mean ± SD from three independent experiments. d The percentage of apoptotic cells was determined by flow cytometric analysis. The data represent the mean ± SD from three independent experiments. e Representative TUNEL staining (red fluorescence) of MDA-MB-468 cells transfected with si-PRLB. Columns are the average of three independent experiments. f Transwell migration and invasion assays were used to evaluate MDA-MB-231 and MDA-MB-468 cell numbers with or without lncRNA-PRLB downregulation. Columns are the average of three independent experiments. g EMT-related markers were detected by Western blot in MDA-MB-231 cells lines with and without lncRNA-PRLB downregulation. *P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t test; NS nonsignificant