Fig. 5.

Improving astaxanthin production using MHP. a Agar plates of astaxanthin strains (the two plates above were different astaxanthin strains) and parental β-carotene strains (the plate below with yellow colonies). b Titers and cell density of different strains chosen from library I, II, and III. Strains Z1-Z6 were picked colonies from library I (crtZ RBS library), where Z2 was a yellow colony (control 1) and others were red colonies. Strains W1–W6 were picked red colonies from library II (crtW RBS library). Strains ZW 1–24 were picked colonies from library III (crtZ and crtW RBS libraries), where ZW12 was a yellow colony (control 2) and the rest were red colonies. c The 3-dimensional correlation of astaxanthin production with the translation rates of crtZ and crtW. d Scatterplot of astaxanthin production and RBS strength of crtZ or crtW