Fig. 8.

MHP for the production of universal compounds. a Demonstration of design strategy of MHP. Globally, we used 3 promoters to regulate the transcription of three major modules: the upstream MVA pathway (module AHT), the downstream MVA pathway (module MPPI), the terpenoid pathway (module NG including nls and GPPS or module NA including nls and ispA). Locally, we used 3 RBSs to control the relative translation of nls and GPPS (or ispA). In total, there were 3⁵ or 243 combinations. b Nerolidol production and cell density for the chosen 54 strains including one control (Ner1, highlighted by an arrow). c Linalool production and cell density for the chosen 54 strains including one control (Lin1, highlighted by an arrow). Nls, a bi-functional nerolidol/linalool synthase