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. 2018 May 11;9(5):566. doi: 10.1038/s41419-018-0615-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative western blot images showing the protein expression levels of AGER when ADSCs treated with different concentration of AGEs. b qPCR analysis of mRNA levels of AGER after cells treated with different concentration of AGEs. c Intracellular ROS production was observed under the fluorescence microscope. d The level of DCF-sensitive ROS was examined by a flow cytometer. e ADSCs were stained with annexin V-FITC/PI and immediately analyzed by flow cytometry. f Western blot analysis of protein levels of caspase-3 and PARP. Every experiment repeated at least three times. Error bars indicate mean ± SD (*P < 0.05)