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. 2018 May 11;9(5):566. doi: 10.1038/s41419-018-0615-9

Fig. 5. miR-5591-5p suppresses AGEs/AGER axis-mediated ROS generation and apoptosis in ADSCs.

Fig. 5

a qPCR analysis of mRNA levels of miR-5591-5p and AGER after cells were transfected with miR-5591-5p mimics with or without AGEs. b Western blot shows the protein expression of AGER after cells were transfected with miR-5591-5p mimics with or without AGEs. c Intracellular ROS generation was visualized under the fluorescence microscope after cells were transfected with miR-5591-5p mimics. The scale bars represent 100 μm. d The level of DCF-sensitive ROS was measured by a flow cytometer after cells were transfected with miR-5591-5p mimics. e Treated ADSCs were stained with annexin V-FITC/PI and immediately analyzed by flow cytometry after cells were transfected with miR-5591-5p mimics. f Western blot analysis of protein levels of caspase-3 after cells were transfected with miR-5591-5p mimics with or without AGEs. g The mRNA levels of miR-5591-5p and AGER after cells were transfected with miR-5591-5p inhibitors with or without AGEs. h Western blot assay of AGER protein levels after cells were transfected with miR-5591-5p inhibitors with or without AGEs. i Intracellular ROS generation was observed under the fluorescence microscope after given miR-5591-5p inhibitors. The scale bars represent 100 μm. j The level of DCF-sensitive ROS was detected by a flow cytometer after cells were transfected with miR-5591-5p inhibitors. k Treated ADSCs were stained with annexin V-FITC/PI and analyzed by flow cytometry after cells were transfected with miR-5591-5p inhibitors. l Western blot analysis of protein levels of caspase-3 after cells were transfected with miR-5591-5p inhibitors with or without AGEs. Data are shown as means ± SD of three replicates (*P < 0.05; **P < 0.01; ***P < 0.001)