Fig. 2. Docetaxel-resistant cancer cells acquired cancer stem cell (CSC) characteristics.
a The CSC sub-population. DU145 and DU145-DR cells were labeled with fluorescent antibodies against CD44 (APC) and CD133 (PE). CD44highCD133high cells were detected by flow cytometry. b The same number of DU145 and DU145-DR cells were plated in soft agar, and cell sphere formation was observed 21 days after cell seeding. The spheres were counted in five randomly selected fields, and the difference of spheres in two groups was compared by statistical analysis. c The protein levels of CSC-associated molecules such as ALDH1, SOX2, and SHH were tested in DU145 and DU145-DR cells by Western blot. d The protein levels of ALDH1, SOX2, and SHH were tested in CNE2 and CNE2TR cells by Western blot