Fig. 4. UHRF1 depletion re-sensitized cancer cells to docetaxel.

a DU145-DR cells were infected with lentivirus-delivered shRNA-UHRF1 or shRNA-CTR, followed by puromycin drug selection. The IC50 values of docetaxel were compared between DU145-DR cells expressing the control shRNA and sh-UHRF1. b Tumor xenografts were established in immune-deficient nude mice with DU145-DR or UHRF1-depleted DU145-DR cells. Nude mice bearing tumor xenografts were treated with or without docetaxel (20 mg/kg) by i.p. injection once a week. The sizes of tumor xenografts were measured every 3 days. c The tumor masses were harvested from immune-deficient nude mice at the experimental endpoint. d The weight of tumors in each group was averaged and compared. e The tumor masses were immersed in embedding reagent OCT and frozen in liquid nitrogen. The frozen tissues were sectioned and stained with a One Step TUNEL Apoptosis Assay Kit. The nuclei were stained with DAPI. The TUNEL-positive red fluorescent cells were observed by fluorescent microscopy with 550 nm excitation and 570 nm emission wavelengths