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. 2018 May 11;9(5):562. doi: 10.1038/s41419-018-0631-9

Fig. 7. FOXM1 regulates uhrf1 gene transcription.

Fig. 7

a FOXM1 regulates uhrf1 gene transcription, but not through the E2F pathway. FOXM1 was depleted with siRNA in DU145 cells, and the mRNA levels of uhrf1, e2f1, e2f2, e2f3, and e2f8 genes were tested by RT-PCR. The protein expression of E2F1 and E2F3 was tested when FOXM1 was depleted with siRNA. b FOXM1 was depleted with siRNA in PC-3 cells, and the mRNA levels of uhrf1, e2f1, e2f2, e2f3, and e2f8 genes were tested by RT-PCR. The protein expression of E2F1 and E2F3 was tested when FOXM1 was depleted with siRNA. c The depletion of FOXM1 with siRNA in HEK-293T cells decreased uhrf1 gene promoter activity. d FOXM1 directly binds to uhrf1 gene promoter. The FOXM1 protein-bound DNA in HEK-293T and CNE2TR cells was purified by ChIP, and primers spanning the FKH binding motif at the uhrf1 gene promoter were designed. FOXM1 protein binding to the uhrf1 gene promoter was detected by ChIP-PCR