Fig. 1. Detection of a specific cleavage of SAS-6.

a HeLa cells were transfected with pSAS-6-Venus-Flag, along with siCTL or siSAS-6. SAS-6^R-Flag includes silent mutations resistant to siSAS-6. Forty-eight hours later, the cells were subjected to immunoblot analyses with antibodies specific to SAS-6 and Flag. b HeLa cells were treated with thymidine for 24 h and transferred into a fresh medium with or without MG132. The cells were harvested at the indicated time points and subjected to immunoblot analyses with antibodies specific to SAS-6, pericentrin (PCNT), PARP-1, caspase-3, and GAPDH. Pericentrin cleavage normally occurs during mitotic exit¹⁴ and the cleavage band was labeled as R2231 cleaved