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. 2018 May 11;9(5):571. doi: 10.1038/s41419-018-0632-8

Fig. 3. Identification of the specific cleavage site of SAS-6.

Fig. 3

a Immunoblot analysis was performed with HeLa cells expressing the wild type, the truncated (Δ511–530 and Δ516–520), or the substituted (D517A) mutant of SAS-6-Flag. The ectopic SAS-6 proteins were detected with antibodies specific to SAS-6 and Flag. b SAS-6-stable cell lines of the wild type and the D517A mutant. It is of note that the recombinant SAS-6 expresses a 3xFLAG at its C-terminus with the Venus tag removed. While the wild type generated a cleaved C-terminus fragment, the D517A mutant was resistant to the cleavage in the presence of MG132. c Immunostaining analysis of the wild type and the D517A mutant of ectopic SAS-6 proteins was performed with the Flag antibody. Scale bar, 10 μm