Fig. 7. Inhibition of the proteasome activity affects the efficiency of centriole duplication in PLK4^Δ24 cells.

a HeLa cells were treated with thymidine for 24 h, and transferred into the medium with MG132. Expression of PLK4^Δ24 was induced with doxycycline at the same time. At the indicated time points, the cells were coimmunostained with antibodies specific to centrin-2 (green) and SAS-6 (red). DNA was stained with DAPI (blue). Scale bar, 10 μm. The number of centrioles per cell were determined with the centrin-2 signals. b The PLK4^Δ24-expressing cells were treated with IDN-6556 and their centriole numbers were counted. Greater than 100 cells per experimental group were analyzed in two independent experiments