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. 2018 Feb 15;9:142–152. doi: 10.1016/j.omtm.2018.02.004

Figure 2.

Figure 2

Analysis of Primary Transplanted Mice

(A) Engraftment of transplanted cells based on human CD46 expression on PBMCs measured by flow cytometry. Each symbol is an individual animal. (B) Engraftment rates in blood, spleen, and bone marrow mononuclear cells analyzed at week 12 after transplantation. (C) Percentage of human γ-globin-positive cells in peripheral blood cells measured by flow cytometry. (D) Fold increase in γ-globin mRNA in total peripheral blood cells in four treated mice (#1–#4) compared to β-YAC mice. (E–H) Analysis of week 12 bone marrow samples. (E) γ-globin expression in erythroid (Ter-119+) and non-erythroid (Ter-119−) cells. The right panel shows a representative sample. (F) Mean fluorescence intensity (MFI) of γ-globin in Ter-119+ and Ter-119− cells. (G) Percentage of human γ-globin mRNA relative to mouse adult β-like globin RNA. Controls are bone marrow samples from untreated C57BL/6 mice. (H) HPLC data. Percentage of human γ-globin protein relative to mouse adult α- and β-globin protein.