Figure 5.

Gigaxonin Expression Persists in Aged Mice IT-Injected with AAV9/JeT-GAN

Gigaxonin gene expression was assessed by measuring the codon-optimized human gigaxonin mRNA in each tissue sample from GAN/Y mice injected at 12 months of age and harvested at 24 months of age (cohort 2) (A) and from GAN/J mice injected at 14–16-months of age and harvested at 24 months of age (cohort 3) (B). Mouse β-actin mRNA was measured as an internal control. β-actin was detected in all samples, and codon-optimized human gigaxonin mRNA was not detected in vehicle-injected animals. Data are means ± SEM for each group (GAN/Y: n = 2 per group; GAN/J: n = 3 per group). Multiple unpaired Student’s t tests were performed on GAN-treated animals, *p < 0.05.