Figure 6.

AAV9/JeT-GAN Reduces IF Inclusions in the DRG of GAN/Y KO Mice

Representative images of light microscopic evaluation of lumbar DRG in 24-month-old normal control (A), vehicle IT-injected GAN /Y KO mice (B), and AAV9/JeT-GAN IT-injected GAN/Y KO mice (C). H&E staining shows unremarkable DRG neurons in control mice (A) versus abundant, brightly eosinophilic inclusion-bearing neurons of vehicle-treated GAN/Y KO mice (B). Neuronal inclusions in GAN-treated mice were significantly reduced compared to vehicle-treated GAN KO mice (C and D). Scale bar represents 61 μm. Arrows indicate neuronal inclusions. Data was analyzed by unpaired Student’s t test.