Fig. 2.

The effect of oligomeric Aβ42 and PJ-34 treatment on gene expression of nuclear and cytosolic sirtuins. PC12 cells were incubated in the presence of oligomeric Aβ42 (AβO, 1 μM) and PJ-34 (20 μM) for 24 h (a–c). The levels of mRNA of nuclear Sirt1 (a), Sirt6 (b), and cytosolic Sirt2 (c) were analyzed via quantitative RT-PCR. The results of RT-PCR were normalized to Actb gene expression. Data represent the mean value ± SEM for three independent experiments with 3–4 replications. ***p < 0.001; **p < 0.01; *p < 0.05—the difference, that was statistically significant compared to the control cells, using one-way analysis of variance (ANOVA) with Neuman-Keuls post hoc test