Fig. 3.

The influence of PARP1 inhibitor PJ-34 and oligomeric Aβ42 treatment on gene expression of mitochondrial sirtuins. PC12 cells were incubated in the presence of oligomeric Aβ42 (AβO, 1 μM) and PJ-34 (20 μM) for 24 h (a–c). The levels of mRNA of mitochondrial sirtuins—Sirt3 (a), Sirt4 (b), and Sirt5 (c)—were analyzed via quantitative RT-PCR. The results of RT-PCR were normalized to Actb gene expression. Data represent the mean value ± SEM for three independent experiments with three replications. ***p < 0.001; **p < 0.01—the difference, that was statistically significant compared to the control cells, using one-way analysis of variance (ANOVA) with Neuman-Keuls post hoc test