Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 7;55(6):5269–5281. doi: 10.1007/s12035-017-0755-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 3 — hSod1 mutants are preferentially directed to the JUNQ compartment in H4 cells. Colocalization of the hSod1 mutants with the VHL protein. As a control, H4 cells were transfected with pcDNA vector. Inclusions were visualized by expression of BiFC-tagged Sod1 (green) and JUNQ was observed by expression of VHL-mCherry (red), nuclei in all experiments were visualized with Hoechst staining. Representative pictures of BiFC. hSod1 A4V (a), L38V (b), G93A (c), and G93C (d) colocalizing with JUNQ. The inclusions formed by BiFC-tagged hSod1 A4V, L38V, and G93C are clearly visualized when proteasome is functional, whereas G93A inclusions are more observed into JUNQ after proteasome inhibition with MG132. Scale bar 20 μm. Magnification ×500