Fig. 6.

The G93A hSod1 mutation increases DNA damage in H4 cells. a Representative images of comet assay. Prestained comets with propidium iodide (PI) were imaged using fluorescence microscopy and tail moments were calculated using the Comet Score software. Scale bar: 40 μm. Magnification: 250X. b Levels of DNA damage in H4 cells expressing BiFC-tagged Sod1 WT and mutants. Quantification of double- and single-strand DNA breaks was made using alkaline comet assay method. BiFC-tagged hSod1expressing cells as well as control cells transfected with empty vector were all examined using the overnight alkaline method to analyze DNA damage. Data is expressed as mean ± SD of at least three replicates. One-way ANOVA, with Bonferroni correction, was used for statistical analysis between WT hSod1 and mutants hSod1 cells with significance level of an asterisk, *** p < 0.05