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. 2018 May 11;18:27. doi: 10.1186/s12896-018-0441-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Constructed engineered mutants with different production capacities. a Oxygen-dependent VB₁₂ biosynthetic pathway in S. meliloti. The branched biosynthesis of heme is also depicted. b The concentration of VB₁₂ in producer strains in fermentation broth was determined by HPLC. Different colors represent the different strains. Values shown are HPLC-determined and are the means of three independent fermentation cultures for each strain. c the amount of intracellular VB₁₂ in recombinant strains in (b)