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. 2018 May 11;9:131. doi: 10.1186/s13287-018-0876-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Propagation of in-vitro aging MSCs. a Numbers of cell population doubling (NCPD) decreased by 69% and 41% from P3 to P8 in the DMEM and αMEM groups, respectively. b As a result of the reduced proliferative capability, MSC population doubling time (CPDT) increased over time in both groups. Numbers represent the mean values. n = 5–8; *p < 0.05, versus the corresponding P8-MSCs cultured under the same expansion condition. αMEM, minimum essential medium alpha; DMEM, Dulbecco’s modified Eagle’s medium