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. 2018 May 11;9:136. doi: 10.1186/s13287-018-0879-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Immunofluorescence analysis of inflammation and apoptosis. Representative images of Iba-1 (a-d) and TUNEL (e-h) immunostained longitudinal sections from the control, single injhection (SI), repeated injections at 3-day intervals (3RI) and repeated injections at 7-day intervals (7RI) groups. Quantitative analysis of the Iba-1⁺/4,6-diamino-2-phenyl indole (DAPI)⁺ (i) and TUNEL⁺/DAPI⁺ (j) cell numbers in the epicentre of the lesion (***p < 0.001, ****p < 0.0001 versus the other three groups; ^#p < 0.05, ^##p < 0.01 versus the 3RI group). The results show that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) transplantation inhibited microglial/macrophage infiltration and apoptosis. Scale bar = 100 μm