Table 1.
Expression level of surface markers analyzed in adipose tissue-derived stem cells (ASC) present in the stromal vascular fraction (SVF) and in expanded ASC
| Markers | Part A | Part B | ||
|---|---|---|---|---|
| ASC in SVF | Expanded ASC | |||
| Fresh | Thawed | 10% FBS | 5% SRGF | |
| CD34 | ++ | ++ | – | – |
| CD45 | – | – | – | – |
| CD31 | – | – | – | – |
| CD90 | ++ | ++ | ++ | ++ |
| CD73 | ++ | ++ | ++ | ++ |
| CD13 | + | + | ++ | ++ |
| CD105 | +− | +− | ++ | ++ |
| CD44 | + | + | + | + |
| CD29 | +− | +− | ++ | ++ |
| CD166 | +− | +− | ++ | ++ |
| CD10 | + | + | + | + |
| HLA I, ABC | + | + | +− | +− |
| HLA II, DR | – | – | – | – |
| CD106 | +− | +− | – | – |
| CD36 | – | – | – | – |
| CD146 | +− | +− | – | – |
| CD235 | – | – | – | – |
| CD144 | – | – | – | – |
| CD11b | – | – | – | – |
| CD11c | – | – | – | – |
| CD14 | – | – | – | – |
Part A reports the expression levels of selected markers in the 34++ 31−SSChigh ASC contained in fresh or thawed SVF samples
Part B reports the expression levels of the same markers in expanded ASC in the presence of 10% fetal bovine serum (FBS) or 5% supernatant rich in growth factors (SRGF) and alpha-minimal essential medium (α-MEM)
Expression levels are described as + and – considering unlabeled cells as reference (see also Fig. 3): ++, bright expression; +, positive expression; +−, dim expression