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. 2018 May 11;9:132. doi: 10.1186/s13287-018-0862-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Interaction of CLR-Ms ATP6AP2/CAV and Gα proteins. hADSCs isolated from healthy subjects and differentiated for 37 days as indicated in Methods. Co-IP of ATP6AP2 and CAV in CLR-Ms: CLR-M fraction of MSCs and NLCs immunoprecipitated (IP) with anti-ATP6AP2 antibody and probed with anti-CAV1 antibody or vice versa (a). Co-IP of ATP6AP2 or CAV with G_α proteins (G_αs, G_αi, G_αq) in CLR-Ms (b). Input for whole experiments were positive. Representative blots of healthy group shown; similar results observed in ND group (data not shown). Ab antibody, CAV caveolin, CTR control, D day, MSC mesenchymal stem cell, NLC neuron-like cell