Fig. 4.

Independent HR events in the adjacent regions containing GT repeats in somatic and germ cells. a Gel electrophoretogram of PCR products amplified with a pair of primers spanning the distal ntl promoter region containing (GT)_n motifs. b Gel electrophoretogram of PCR products amplified with a pair of primers spanning the proximal ntl promoter region lacking (GT)_n motif. M, marker. PC, paternal control. MC, maternal control. H, 4-day old heterozygous embryos. c, d The linkage patterns of the paternal and maternal genetic makers in the flanking sequences of the three (GT)_n motifs in the 4-day old embryos and in the sperm of heterozygote, respectively. Black lines represent the conserved regions. Orange lines, dotted lines and arrow heads represent the paternal genetic markers. Blue lines, dotted lines and arrow heads represent the maternal genetic markers. Orange and blue boxes represent the paternal and maternal (GT)_n motif 1, respectively. Gray boxes represent the contracted or expanded (GT)_n motif 1. PD, paternal-derived wild type clones. MD, maternal-derived wild type clones. Crossovers I, II, I + II, crossover types. Numbers of the clones are indicated at the right. Note that the exchanged fragments in the co-occurrence of crossovers I and II are identical to the sequence regions from one of the crossover I sites to one of the crossover II sites