Fig. 1.

Graphical presentation of the laboratorial design. A float tray substrate was positioned in plastic pots reserved for two experiments (transcriptomic analysis and metabolomics analysis). The seeds were grown in a greenhouse with supplemental lighting (16 h light/8 h dark cycle, 26 ± 3 °C day/night). When the seeds were the two true leaves stage inoculated nematodes. There are three biological replicates in transcriptomic analysis and six biological replicates in metabolomics analysis. Per replicate of two plant roots is one sample. At 5 day post inoculation (dpi), roots samples were rapidly harvested, frozen in liquid N₂, and stored at − 80 °C for further experiments