Fig. 5.

Chronological analysis of p-α-Syn pathology after inoculation of α-Syn preformed fibrils. a Number of p-α-Syn–positive neurons in the dmX of mice bilaterally examined at 45 days, 4 months, 8 months, and 12 months postinoculation of α-Syn preformed fibrils (n = 5–8). One-way ANOVA with Tukey’s multiple-comparisons test was performed; *p < 0.05, ***p < 0.001, n.s. means not significant. b p-α-Syn (EP1536Y) immunohistochemistry in the dmX of a mouse at 12 months postinoculation. Dashed line outlines the approximate area of the dmX. dmX, dorsal motor nucleus of the vagus nerve; 4V, fourth ventricle. Scale bar 100 μm. A p-α-Syn–positive neuron (arrow) is enlarged in the inset. Scale bar 20 μm. c p-α-Syn (EP1536Y) immunohistochemistry in the myenteric plexus of a mouse at 12 months postinoculation. Scale bar 100 μm. A p-α-Syn–positive neuron (arrow) is enlarged in the inset. Scale bar 20 μm. Data are the mean ± SEM