Fig. 5.

TGF-β1 regulates the interaction of STAT1α/STAT1β with ALK1/ALK5/TβRII. HEK-293 T cells were transiently co-transfected with STAT1α-myc or STAT1β-myc and ALK1-HA, ALK5-HA or TβRII-HA plasmids in the absence or presence of TGF-β1 (10 ng/ml) for 24 and 48 h. a Immunoprecipitation (IP) with anti-myc antibody for STAT1α-myc or STAT1β-myc and immunoblotting (IB) with anti-HA antibody for ALK1-HA. Cell lysates of input samples were used for detecting the expression of STAT1-myc, ALK1-HA, and β-actin. b Densitometric and semi-quantitative analyses of the gels of IP in (a). c IP with anti-myc antibody for STAT1α-myc or STAT1β-myc and IB with anti-HA antibody for ALK5-HA. Cell lysates of input samples were used for detecting the expression of STAT1-myc, ALK5-HA, and β-actin. d Densitometric and semi-quantitative analyses of the gels of IP in (c). e IP with anti-myc antibody for STAT1α-myc or STAT1β-myc and IB with anti-HA antibody for TβRII-HA. Cell lysates of input samples were used for detecting the expression of STAT1-myc, TβRII-HA, and β-actin. f Densitometric and semi-quantitative analyses of the gels of IP in (e). n = 3 independent experiments; *, P < 0.05