Fig. 3.

Maternal diabetes and Hif1a mutation affect the transcriptome of the LV of Hif1a^+/− offspring. The global gene expression profile of LVs isolated from the hearts of 12-week-old Wt and Hif1a^+/− mice from diabetic or non-diabetic pregnancy (n = 3 LVs from mice of different litters per group) was determined by RNA sequencing. a Hierarchical clustering of differentially expressed genes in all groups are presented as a heat map and dendrogram. b Classification of differentially expressed genes affected by Hif1a^+/− mutation and exposure to maternal diabetes by functional annotation tool DAVID. Values represent number of genes per each category. c Venn diagram shows the number and proportion of differentially (up- and down-regulated) expressed genes with ≥ 30% change in Hif1a^+/− and Wt from diabetic pregnancy and Hif1a^+/− from non-diabetic pregnancy vs. Wt from non-diabetic pregnancy. d Enrichment analysis using g:Profiler; values represent number of genes per each biological process category