Fig. 4.

Glucose consumption and lactate secretion rates and response to mitochondrial complexes inhibitors in GOT1-null 143B cells. a Glucose concentrations, b Glucose consumption rates, c Lactate concentrations, d Lactate secretion rates determined in culture supernatants after 2 days (dividing cells) and 4 days (confluent cells). Cell viability in cells grown in complete medium for 2 days and then grown for 24 h in medium e without glucose only or supplemented with 10 mM pyruvate (Pyr), f with 0 or 1 μM antimycin A, g with 0 or 1 mM (TTFA). All experiments have been repeated 3 times independently. Data are presented as mean ± s.d. Unpaired student’s t-test was performed. *** p < 0.001; ** p < 0.01; * p < 0.05