Fig. 1.

LV-CXCL12 virus successfully transfected EPCs in vitro. A Clone of pLV-CXCL12-IRES-GFP plasmid by inserting mouse cxcl12 cDNA sequence in pLV-IRES-GFP plasmid, then cotransfected 293 T cells with pVSVG and pDelta plasmid to package LV-CXCL12 and LV-GFP virus. B Flow cytometry to characterize human umbilical cord blood-derived EPCs by cell surface markers CD31, KDR, CD34, and CD133. C LV-CXCL12-transfected EPCs in bright field (left), fluorescent field (middle), and flow cytometry (right) to identify transfection efficacy of CXCL12-EPCs. Scale bar: 100 μm. D Real-time PCR and western blot analysis to detect CXCL12 mRNA (a) and protein (b) expression in EPCs, GFP-EPCs, and CXCL12-EPCs. n = 3, technical replica. Data presented as mean ± SD. **p < 0.01. GFP green fluorescent protein, EPC endothelial progenitor cells, GFP-EPC endothelial progenitor cell modified by gfp gene, CXCL12-EPC endothelial progenitor cell modified by cxcl12 gene