Fig. 8.

JQ1 can rescue the splicing activity lost by Tat mutants. HEK293T cells were transfected with the pLTR.gp140/EGFP.Rev∆38/DsRed splicing reporter and 100 ng of pTat101 (AD8) WT or with specific mutations (K28A, K50A, K50/51A, K71A and R53A). Cells were treated with DMSO (D, 1:5000) or JQ1 (J, 1 μM), harvested at 48 h and DsRed and EGFP expression quantified using flow cytometry. a Data were represented as the proportion of spliced product [DsRed/(DsRed + EGFP) × 100] relative to WT Tat+ DMSO. Comparisons between DMSO and JQ1 for either no Tat, WT Tat or each mutant Tat were made using multiple paired T tests. b The difference between JQ1 and DMSO in the proportion of spliced product [DsRed/(DsRed + EGFP) × 100] was calculated for each Tat mutant, no Tat or WT Tat, and then compared to the difference with WT Tat101. Only statistically significant comparisons are shown *p < 0.05; **p < 0.01; ***p < 0.001. The black lines represent the mean ± SEM (n = 5)