Table 1.

List of primer sequences used in this study

Target genes	Forward primer (5’–3’)	Reverse primer(5’–3’)	Annealing temperature (°C)
GAPDH	ATGGTGAAGGTCGGTGTGAACG	TTACTCCTTGGAGGCCATGTAG	55–62
P0	GCTCTTCTCTTCTTTGGTGCTGTCC	GGCGTCTGCCGCCCGCGCTTCG	57
NGFR p75	ATGAGGAGGGCAGGTGCTG	TCACACTGGGGACGTGGC	55
GFAP	GGACATCGAGATCGCCACCTACAG	CATCCCGCATCTCCACCGTCTTTAC	57
NGF	ATGTCCATGTTGTTCTACAC	TCAGCCTCTTCTTGCAGCC	56
BDNF	ATGACCATCCTTTTCCTTAC	CTATCTTCCCCTTTTAATGG	57
CNTF	ATGGCTTTCGCAGAGCAAA	CTACATCTGCTTATCTTTGGCC	56

BDNF, brain-derived neurotrophic factor; CNTF, ciliary neurotrophic factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFAP, glial fibrillary acidic protein; NGF, nerve growth factor; NGFR p75, nerve growth factor receptor p75; P0, myelin protein zero