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Cell-free prototyping of a nonmodel microbe. (A) Testing of synthetic gene-expression plasmids in NCF using endogenous energy regeneration and transcription–translation components. (B) Parallel transcription–translation measurements with the MGapt (mRNA) aptamer and GFP in B. megaterium NCF. (C) A semiautomated workflow incorporating liquid-handling robotics for rapid screening of cell-free reactions (RXNs).
