Fig. 3.
Coexpression of soybean NSFs reduces cell death symptoms caused by α-SNAPRhg1LC; NSFRAN07 gives strongest protection. (A) N. benthamiana leaves ∼6 d after agroinfiltration with 9:1 or 14:1 strain mixture (9 or 14 parts Agrobacterium that delivers LC [α-SNAPRhg1LC] to one part Agrobacterium that delivers the indicated soybean NSF [NSFCh07, NSFRAN07, NSFCh13] or EV control). (B) Scoring of cell death severity, across multiple independent experiments, in N. benthamiana leaf patches coexpressing NSFCh07, NSFRAN07, or NSFCh13; n is number of leaves scored; error bars show SEM. (C) Like A, but 7:1 or 11:1 mixed cultures expressing α-SNAPRhg1LC with NSFRAN07, NSFN.benth, NSFCh13, or empty vector. (D) Silver-stained SDS/PAGE of recombinant NSFN.benth bound in vitro by recombinant WT, LC, or HC Rhg1 α-SNAP proteins or WT α-SNAP lacking the final 10 C-terminal residues (WT1–279).