Fig. 1.

cifA rescues cytoplasmic incompatibility when it is highly expressed throughout oogenesis. (A) Hatch rate assays were conducted with transgenic expression of cifA under the control of nos-GAL4-tubulin or MTD-GAL4 drivers. Each dot represents a replicate. Rescue occurred only under MTD-GAL4 expression. Horizontal dotted lines from top to bottom separate cross-types with CI, cifA expression, and rescue. Wolbachia infections are represented by filled sex symbols, and expressed genes are noted to the right of the corresponding sex. n = 27–59 for each experimental cross across two experiments (both shown). Vertical bars represent medians, and letters to the right indicate significant differences based on α = 0.05 calculated by Kruskal–Wallis and Dunn’s test for multiple comparisons. (B) Gene expression fold change of cifA relative to the Drosophila housekeeping gene rp49 was determined on a subset of abdomens from females expressing cifA via MTD-GAL4 or nos-GAL4-tubulin with 2^−∆∆Ct. Horizontal bars represent medians with 95% confidence intervals, and letters above indicate significance based on a Mann–Whitney U test. In both cases, statistical comparisons are between all groups. Exact P values are provided in Table S2. Hatch rate experiments testing expression of cifA under MTD-GAL4 or nos-GAL4-tubulin have been repeated four and five times, respectively.