Fig. 2.

Rescue of cytoplasmic incompatibility is specific to cifA. Hatch rate assays were conducted with transgenic expression of cifA, cifB, and cifA;B using the MTD-GAL4 driver for expression throughout oogenesis. Each dot represents a replicate. Wolbachia infections are represented by filled sex symbols, and expressed genes are noted to the right of the corresponding sex. n = 11–29 for each experimental cross. Vertical bars represent medians, and letters to the right indicate significant differences based on α = 0.05 calculated by Kruskal–Wallis and Dunn’s test for multiple comparisons. Statistical comparisons are between all groups. Exact P values are provided in Table S2. Hatch rate experiments testing expression of cifA under MTD-GAL4 have been repeated four times.