Fig. 4.

Averaged alignment residual as a function of the acquired image. Averaged residual calculated from 2300, 17645 and 25624 individual residuals collected over the tilt-series from the ribosome (top), proteasome (middle) and Basal Body (bottom) datasets. Tilt-series acquisition was done in two branches that are marked in the plots. The green and black curves present the residuals from the alignment considering motion, using bivariate and trivariate polynomials, and the red one corresponds to the standard alignment. Here the original tilt angles from the goniometer were preserved.