Table 1.

Test datasets. Details and summary of results.

	Ribosome	Proteasome	Basal body
Acquisition details
N. tilt-series:	7	14	6
Tilt range, interval:	$\pm 60°\text{,}3°$	$\pm 60°\text{,}3°$	$\pm 60°\text{,}1°$
N. images/tilt-series:	41	42	121
N. frames/image:	3	5	5
Accumulated dose:	$60{\text{e}}^{-}/{\AA }^2$	$60{\text{e}}^{-}/{\AA }^2$	$60{\text{e}}^{-}/{\AA }^2$
Defocus:	3–5 $\mathrm{\mu }\text{m}$	1–4 $\mathrm{\mu }\text{m}$	1–4 $\mathrm{\mu }\text{m}$
Pixel size:	2.17 $\AA$	2.56 $\AA$	4.82 $\AA$
N. fiducials:	6–11	26–55	22–43
N. subtomograms:	3120	3928	1980
Results of alignment
Avg. mean residual (standard alignment)	1.95	1.83	1.68
Avg. mean residual (bivariate motion)	0.75	0.78	1.36
Avg. mean residual (trivariate motion)	0.62	0.63	0.99
Avg. ratio measures/unknowns (bivariate motion)	1.42	7.39	5.88
Avg. ratio measures/unknowns (trivariate motion)	1.15	4.43	3.53
Avg. LOO residual (bivariate motion)	4.34	0.92	1.66
Avg. LOO residual (trivariate motion)	4.18	1.00	1.50
Results of subtomogram averaging
Resolution (standard alignment):	13.8 $\AA$	12.0 $\AA$	30.4 $\AA$
Resolution (new alignment):	12.6 $\AA$	9.0 $\AA$	29.0 $\AA$