Comparison of net photosynthetic rate (●, ○), CO2 uptake (▾, ▿), and HCO3− uptake (▪, □) for Symbiodinium sp. freshly isolated from the giant clam T. gigas (black symbols) and a 2-d-old culture (white symbols) with differing Ci concentrations. Assays were conducted in 25 mm BTP (pH 8.0), 428 mm NaCl, and 50 μm AZA at 28OC with a photon flux density of 500 μE m−2 s−1. Data were collected using the mass spectrometry disequilibrium technique described in “Materials and Methods.”