Alpha-toxin levels in mouse lungs (top) and bacterial burden (bottom) at 8h post-infection. Mice were infected with ~5 × 107 CFU of wild-type S. aureus Newman, agrA C55S C199S (nitric oxide-resistant mutant), or ΔagrA (null). Alpha-toxin measurements were determined on lung homogenates by ELISA.
Individual values are plotted with horizontal lines showing medians of groups, Mann-Whitney U test, *p<0.05 (n = 20).
See also Figure S7.