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. 2018 Apr 29;2018:3093516. doi: 10.1155/2018/3093516

Figure 5.

Figure 5

Effects of Huanglian-Wendan decoction on NF-κB and NLRP3 inflammasome activation in the hippocampus of CUMS rats. (a) The nuclear entry of NF-κB was quantitated by the nuclear/cytoplasmic ratio of NF-κB protein levels normalized by lamin A/C or GAPDH, respectively. (b) The relative protein levels of p-NF-κB were normalized to NF-κB. (c) The relative protein levels of p-IκBα were normalized to IκBα. (d) The relative protein levels of NLRP3 were normalized to β-actin. (e) The relative protein levels of ASC were normalized to β-actin. (f) The relative protein levels of mature Caspase-1 were normalized to β-actin. (g) The relative protein levels of mature IL-1β were normalized to pro-IL-1β. Protein levels of p-NF-κB, NF-κB, p-IκBα, IκBα, NLRP3, ASC, mature Caspase-1, mature IL-1β, pro-IL-1β, and β-actin were analyzed by the Western blot method. The data were expressed as fold of control values. All data were expressed as mean ± S.E.M. (n = 4–8). #P < 0.05 and ##P < 0.01 compared with the non-CUMS group; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with the CUMS group.